Fig 1: The Spearman's relationship between HDL lipids and the level of ANGPTL3. (A–C) The correlation of HDL-C and RC in a different time. (A) Fasting; (B) 2 h after a meal; (C) 4 h after a meal. (D) The Spearman's correlation of post-prandial changes in HDL-C and RC.
Fig 2: Post-prandial change of glucose may affect the ANGPTL3 expression. (A) Eight-week-old male WT mice were fasted for 16 h, or fasted for 16 h and then refed a high-carbohydrate, low-fat diet for 3 h. The blood glucose levels in mice were measured (n = 4 per group). (B) The blood lipids profiles in mice (n = 4 per group) treated as in (A). (C) qPCR analysis of the Angptl3 mRNA in mice livers (n = 4 per group) treated as in (A). (D) Relative expression of ANGPTL3 in HepG2 cells, which were fasted for 12h, and then treated with 0, 5, and 25 mM glucose for 12 h (n = 3 per group). (E) Relative expression of ANGPTL3 in HepG2 cells transfected with ChREBP siRNA or control in different glucose concentrations (n = 3 per group). *p = 0.05; **p < 0.01; ***p < 0.001; Statistical significance was determined using unpaired two-tailed Student's t-test (A–C) and two-way ANOVA with multiple comparisons (D,E). Bars represent mean ± SEM.
Fig 3: Changes in levels of ANGPTL3 at 2 and 4 h after a meal. (A) In all subjects (n = 49). (B) Changes in levels of ANGPTL3 at 2 and 4 h after a meal, according to ?TG. *p = 0.05 when compared with 2 h post-prandial, #p = 0.05 when compared with fasting. Statistical significance was determined using Generalized Estimating Equations. Bars represent mean ± SEM.
Fig 4: Schematic diagram of betatrophin and angiogenin-likeprotein3 participating in lipid regulation. Betatrophin synthesized by the liver under the stimulation of feeding or insulin can promote the cleavage of angiogenin-likeprotein3 (ANGPTL3) to release the active N-terminal catalytic core domain (CCD) domain, which leads to a decrease in the inhibitory effect of ANGPTL3 on Lipoprotein lipase (LPL) activity and indirectly promotes LPL to participate in lipid metabolism. When triglyceride- derived fatty acids enter the adipocytes, some of them form lipid droplets, while the other part induces the increase of LPL synthesis in adipocytes, and enters the blood circulation to participate in lipid metabolism mediated by glycosylphosphatidylinositol high density lipoprotein binding protein 1. GPIHBP1: Glycosylphosphatidylinositol high density lipoprotein binding protein 1; HSPG: Heparan sulfate proteoglycan; CM: Chylomicrons; TG: Triglycerides.
Fig 5: Comparison of ANGPTL3 level in the serum and urine of NS patients and controls. (A) Serum ANGPTL3 level in NS and controls. NS, n = 200; Control, n = 80. (B) Urinary ANGPTL3/Cre level in NS and controls. NS, n = 164; Control, n = 80. Using Mann–Whitney U-test, ****P < 0.0001.
Supplier Page from Abcam for Human ANGPTL3 ELISA Kit